Figure 1.

Poly(dA:dT) treatment decreased the expression of PRINS but induced the expression and secretion of cytokines. Normal human epidermal keratinocytes were transfected with poly(dA:dT) for 12 h with or without priming for 24 h with 5 ng/ml tumor necrosis factor-α (TNF)-α and interferon-γ (IFN-γ). RNA expression was detected for PRINS (A) and cytokines interleukin-6 (IL-6) (B), IL-8 (C), IL-1α (D), IL-1β (E), TNF-α (F) by real-time RT-PCR. Data are presented as mean ± SE of five independent experiments (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).