Figure 3.

Overexpression of PRINS regulates the expression and secretion of interleukin-6 (IL-6) and IL-8. In parallel to priming with 5 ng/ml tumor necrosis factor-α and interferon-γ, normal human epidermal keratinocytes were transfected with a pcDNA3.1(+) vector containing the PRINS cDNA; an empty pcDNA3.1(+) vector was used as control. After 24 h, cells were transfected with poly(dA:dT). RNA expression of cytokines IL-6 (A) and IL-8 (B) was detected by real-time RT-PCR. Secretion of cytokines IL-6 (C) and IL-8 (D), was detected by ELISA from cell supernatants. Data are presented as mean ± SE of four independent experiments (*p ≤ 0.05; **p ≤ 0.01).