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PRINS specifically binds to interleukin-6 (IL-6) mRNA. The binding affinity of PRINS (A) and ΔPRINS (B) to IL-6 mRNA was determined by analyzing the initial fluorescence enhancement caused by specific binding. The concentration of labeled IL-6 mRNA was constant (10 nM), while the concentration of PRINS and ΔPRINS varied from 62 pM to 127 nM.
