Figure 6.

The 538–622 nt region of PRINS is required for the regulation of interleukin-6 (IL-6) expression. In parallel to priming with 5 ng/ml tumor necrosis factor-α and interferon-γ, normal human epidermal keratinocytes were transfected with a pcDNA3.1(+) vector containing the PRINS cDNA or the ΔPRINS cDNA (containing a scrambled version of the IL-6 binding site at positions 538–622 nt); the empty pcDNA3.1(+) vector served as control, after 24 hours cells were transfected by poly(dA:dT). Expression of cytokines IL-6 (A), CCL-5 (B), and IL-8 (C) was detected by real-time RT-PCR. Secretion of cytokines IL-6 (D), CCL-5 (E), and IL-8 (F) was detected by ELISA from cell supernatants. Nucleotide positions based on the AK022045 reference sequence. Data are presented as mean ± SE of three independent experiments (*p ≤ 0.05; **p ≤ 0.01).