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. 2017 Aug 29;8:1040. doi: 10.3389/fimmu.2017.01040

Figure 1.

Figure 1

Binding of recombinant, purified, and native pentraxin 3 (PTX3), C-reactive protein (CRP), or SAP to CCs. (A) Concentration-dependent binding of recombinant PTX3 (rPTX3), purified CRP (pCRP), and purified SAP (pSAP) to CC. Binding was assessed by flow cytometry as MFI and data are given as mean ± SEM (n = 3). (B) Binding of rPTX3, pCRP, or pSAP to CC ± 10 mM EDTA (black/gray). (C–E) Binding of native PTX3, CRP, or SAP from 5% NHP (C), NHS (D) or a pool of serum from three individuals with systemic inflammation (E) diluted in Barbital/BSA ± 10 mM EDTA (black/gray). Isotype controls are shown as white histograms. Histograms represent one of three independent experiments. CCs, cholesterol crystals; NHP, normal human plasma; NHS, normal human serum; MFI, median fluorescence intensity.