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. 2017 Aug 29;8:1040. doi: 10.3389/fimmu.2017.01040

Figure 3.

Figure 3

Pentraxin 3 (PTX3), C-reactive protein (CRP), and C1q interact on the surface of CCs. C1q binding to CC increase binding of PTX3 (A) and binding of PTX3 or CRP increase the binding of purified C1q (pC1q) and native C1q from plasma or serum samples (B–D). (A) Binding of recombinant PTX3 (rPTX3) (2.5 µg/mL), purified CRP (pCRP) (0.02 µg/mL), or purified SAP (pSAP) (0.02 µg/mL) to CC preincubated with 0, 1, or 4 µg/mL pC1q. (B) Binding of pC1q (2 µg/mL) to CC preincubated with rPTX3 (0, 1.25, 2.5 or 5 µg/mL), pCRP (0, 0.01, 0.04, or 1 µg/mL), or pSAP (0, 0.01, 0.04, or 1 µg/mL). (C) Binding of C1q from 5% NHP to CC preincubated with rPTX3 (0, 1.25, 5 or 10 µg/mL), pCRP (0, 0.01, 0.04, or 1 µg/mL), or pSAP (0, 0.01, 0.04, or 1 µg/mL). (D) Binding of C1q from 5% UCS to CC preincubated with rPTX3 (0, 1.25, 5, or 10 µg/mL), pCRP (0, 0.01, 0.04, or 1 µg/mL), or pSAP (0, 0.01, 0.04, or 1 µg/mL). Binding was assessed by MFI and data are given as mean ± SEM (n = 3). *p < 0.05, **p < 0.01 compared with buffer or as otherwise indicated. CC, cholesterol crystals; NHP, normal human plasma; UCS, umbilical cord serum; MFI, median fluorescence intensity.