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. 2017 Aug 29;8:1040. doi: 10.3389/fimmu.2017.01040

Figure 4.

Figure 4

Binding of C-reactive protein (CRP) to CCs increases C1q-mediated complement activation in UCS. Complement activation was measured as C5b-9 deposition on CC and assessed by flow cytometry. (A) C5b-9 deposition on CC incubated with 10% NHP ± C1q inhibitory antibody (C1q Inhib Ab). CC were preincubated with recombinant PTX3 (rPTX3) (0 or 10 µg/mL), purified CRP (pCRP) (0 or 1 µg/mL), or purified SAP (pSAP) (0 or 1 µg/mL). (B) C5b-9 deposition on CC incubated with 10% UCS ± C1q Inhi Ab. CC were preincubated with rPTX3 (0 or 10 µg/mL), pCRP (0 or 1 µg/mL), or pSAP (0 or 1 µg/mL). Binding was assessed by MFI, and data are given as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 compared with buffer or as otherwise indicated. CC, cholesterol crystals; NHP, normal human plasma; UCS, umbilical cord serum; MFI, median fluorescence intensity.