FIGURE 1.

CD63 was incorporated into HIV-1 particles and was required for HIV-1 virion production. (A) CD63 expression in 293T, COS7, and TE671 was analyzed by western blotting using anti-CD63 antibody. (B) 293T cells were transfected using CD63 WT-GFP together with pcDNA3.1 or HIV-1 vector construction plasmids. Cell lysates and virion pellets were analyzed by western blotting. (C) TE671 cells transduced by empty or shCD63-encoding lentivirus vector were transfected using the VSV-pseudotyped HIV-1 vector construction plasmids. Culture supernatants from the transfected cells were used to inoculate TE671 cells. Transduction titers from the empty vector-transduced cells were set to 1 and the relative values ± SD are indicated. Asterisk indicates significant differences. (D) Cell lysates and virion pellets prepared from the transfected TE671 cells were analyzed by western blotting. (E) The p24 levels in cell lysates were normalized against the actin levels, and the p24 levels in virion pellets were normalized against the normalized p24 levels. The normalized p24 levels in the virion pellets from TE671 cells transduced by empty vector cells were set to 1 and the relative values ± SD are indicated. Asterisk indicates significant differences.