Table 4.

Confirmation of Microarray Data by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction

Gene	Fold changea in gene expression: PHHb/HBV vs PHHb/mock
	PHH2	PHH3	PHH4	PHH5
cIAP2	5.1 I	3.9 I	5.1 I	2.9 I
Mn-SOD	3.9 I	2 I	4.1 I	3.4 I
TNFAIP8	2 I	2.3 I	2.3 I	3.1 I
IER3	5.7 I	1.7 I	1.5 I	1.6 I
IGFBP1	116 I	5.3 I	23 I	7.4 I
CRP	18.5 I	115 I	269 I	88 I
CCL2	4 I	21 I	6.7 I	6.9 I
IL-8	24.7 I	10.8 I	8.5 I	4.2 I
APOE	−19 D	−3.5 D	−1.4 D	−2.3 D
CYP3A4	−4 D	−8.6 D	−4.3 D	−12.2 D
2‘5’OAS	nc	−2 D	nc	−1.6 D
IP10	nc	−1.7 D	−1.5 D	−2.5 D

HBV, hepatitis B virus; PHH, primary human hepatocyte.

^aIncreased (I) and decreased (D) compared with mock-infected PHH; mean values of at least 2 independent measurements per experiment are given. nc, no change in gene expression detected.

^bPHH prepared from different patient materials (PHH2–PHH5).