Skip to main content
. 2017 Aug 28;8:1020. doi: 10.3389/fimmu.2017.01020

Figure 2.

Figure 2

In the absence of interleukin-18 binding protein (IL-18BP), CD27+, CD11b− natural killer (NK) cells are increased in abundance while CD27−, CD11b+ NK cells, including terminally differentiated KLRG1-expressors, are reduced in abundance. (A) Representative gating of CD27 versus CD11b on wild type (WT) and IL-18BPKO splenic NK cells. (B) Immature CD27+, CD11b− NK cells are increased in proportion and abundance in the spleens of IL-18BPKO mice, while (C) mature CD27−, CD11b+ NK cells are reduced in proportion and abundance. (D) Representative dot plots showing KLRG1 expression versus either CD27 or CD11b and KLRG1 gating on CD27−, CD11b+ NK cells. KLRG1 expression among NK cells is mostly among the CD27−, CD11b+ compartment. (E) Although the proportion of KLRG1 expressers within the CD27−, CD11b+ compartment was relatively similar between the two genotypes, the overall abundance of KLRG1+, CD27−, and CD11b+ was reduced among IL-18BPKO mice. Results from three unique experiments from six WT and eight IL-18BPKO mice. Bars represent median. Significance calculated with Mann–Whitney U test. ***p < 0.001, ns, not significant.