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. 2017 Aug 28;8:1020. doi: 10.3389/fimmu.2017.01020

Figure 8.

Figure 8

CD27 and CD11b proportions are altered following 24 h culture with interleukin (IL)-18. We cultured splenocytes with IL-18 (50 ng/mL), IL-2 (2 ng/mL) or both IL-18 and IL-2 overnight to observe if IL-18 is capable of altering proportions of CD27 and CD11b-expressing subsets from their proportions immediately following isolation (fresh). (A) Representative results from one wild type (WT) and one IL-18BPKO. (B) IL-18-treated cultures possessed increased proportions of CD27+, CD11b− natural killer (NK) cells and reduced proportion of CD27−, CD11b+ NK cells, an effect similar to what we observed in IL-18BPKO mice. IL-18 in the presence of IL-2 reduced proportions of CD27+, CD11b− NK cells while increasing CD27+, CD11b+ proportions and either decreasing (WT) or having no effect (IL-18BPKO) on CD27−, CD11b+ proportions. Results of three separate experiments, one WT, and one IL18BPKO per experiment. Bars denote mean values and error bars represent SD.