Table 1. Steady-state kinetic parameters of M.SinI.

	GGACCa	GGGCCa	GGICCa
	CCTGG	CCCGG	GGCCC
KmDNA (nM)	17 ± 3	116 ± 20	95 ± 13
kcat (min–1)	0.3 ± 0.054	0.014 ± 0.0035	0.436 ± 0.077
kcat/KmDNA (×105 M–1 s–1)	2.94 ± 0.68	0.02 ± 0.002	0.76 ± 0.23
Relative kcat/KmDNA	1	0.007	0.26

^aSubstrate sequence.