Table 2. Methylation of different substrate sequences by M.EcoRII.
| Extract (fold dilution) | CCAGG | CCGGG | CCIGG |
| |
GGTCC |
GGCCC |
GGCCC |
| pT71-Cys (1×) | 71 646 ± 3850 | 1940 ± 263 | 109 724 ± 6641 |
| pT71-Cys (10×) | 13 336 ± 1562 | 688 ± 166 | 45 932 ± 6157 |
| pT71-Cys (100×) | 1996 ± 301 | 252 ± 35 | 7854 ± 1690 |
| No plasmid (1×) | 189 ± 5 | 195 ± 4 | 200 ± 9 |
In vitro methylation reactions using cell-free extracts of BL21(DE3) carrying pT71-Cys or no plasmid. Values (average of four experiments) indicate incorporated 3H radioactivity in c.p.m.