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. 2001 Aug 1;29(15):3188–3194. doi: 10.1093/nar/29.15.3188

Table 3. Methylation of different substrate sequences by the wild-type and N172S mutant M.SinI.

Clone GGACC GGGCC
 
 CCTGG
 CCCGG
pSin17 (WT) 23 067 ± 4269 63 ± 16
pSin26-19 (Asn172Ser) 1973 ± 714 913 ± 179
pSin7 (WT) 48 661 ± 4342 78 ± 14
pSin27-19 (Asn172Ser) 8713 ± 962 5020 ± 170

In vitro methylation reactions using cell-free extracts. Values (average of four experiments) indicate incorporated 3H radioactivity in c.p.m.