Table 2.
Description of the newly identified RGs, primer sequences, and amplification characteristics.
| Gene ID | Gene locusa | Gene description | Primer sequence (5′–3′) | Product size (bp) | E | R2 |
|---|---|---|---|---|---|---|
| SlFRG01 | Solyc06g005360 | Actin depolymerizing factor 3 | F: TGACAAAGGAAAGTGTCCCA | 133 | 0.76 | 0.994 |
| R: ATCTGAATTCCGTCCAGCTC | ||||||
| SlFRG02 | Solyc01g088040 | Unknown | F: GTTGATGAAGGGAGAGCCAT | 95 | 0.78 | 0.998 |
| R: GAGAGTAGATCACGGCAGCA | ||||||
| SlFRG03 | Solyc02g063070 | Unknown | F: GGCTGAACTGGCTCCTACTC | 109 | 0.77 | 0.999 |
| R: TTTCGCAAGGTTACAAGCAC | ||||||
| SlFRG04 | Solyc12g095990 | RNA helicase DEAD40 | F: ACTCACGGAGACATGGATCA | 107 | 0.78 | 0.999 |
| R: TCAATACCACGAGCAAGGAG | ||||||
| SlFRG05 | Solyc01g104170 | Ankyrin repeat domain-containing protein | F: CAAAGGTTGATGCATTGGAC | 110 | 1.05 | 0.958 |
| R: AGTTACAGCGGCTCCATTCT | ||||||
| SlFRG06 | Solyc11g070030 | Unknown | F: CCACACGACACTTCCATCTC | 111 | 0.90 | 1 |
| R: TCAGCCGGTTTAGATATCCC | ||||||
| SlFRG07 | Solyc01g091150 | Golgi SNAP receptor complex member | F: CAATCAAGAAGGATCCAGCA | 149 | ||
| R: GCCTTGCCAGTTTCTGTGTA | ||||||
| SlFRG08 | Solyc11g042930 | E3 ubiquitin ligase | F: CTGGCTGCCAACTACTTGAA | 71 | 0.82 | 0.983 |
| R: TTAATCATGTCTGCCACGGT | ||||||
| SlFRG09 | Solyc03g031950 | unknown | F: CCCAATTTCTTCCTCCGTAA | 108 | 0.98 | 0.954 |
| R: TGGCCTAAGAAGTCCGAATC | ||||||
| SlFRG10 | Solyc10g078450 | U6 snRNA-associated Sm-like protein | F: TATAGCAATGGAGCAAACCG | 112 | 0.75 | 0.997 |
| R: TGTCCTCTTTGATGTGCTGA | ||||||
| SlFRG11 | Solyc05g023800 | Uncharacterized protein | F: ACAGGCACTGCATGAAGAAG | 109 | 0.91 | 0.993 |
| R: GTGAAACTGATCCGCTCTGA | ||||||
| SlFRG12 | Solyc04g009770 | Ran protein/TC4 protein 1 | F: AATCAAGTCCCAACCTGGAG | 93 | 0.90 | 0.989 |
| R: TTCATGAATGGCCTTTGGTA | ||||||
| SlFRG13 | Solyc01g103450 | DNAJ chaperone | F: AATGACAAGGTTTCCAAGGC | 100 | ||
| R: CTTCTAATCCAGCAATGCGA | ||||||
| SlFRG14 | Solyc06g076970 | Peptidyl-prolylcis-trans isomerase | F: CTCTGCAGTTTGGTCGTGAT | 126 | 0.84 | 0.983 |
| R: AATACGACCGGCAGGTTTAC | ||||||
| SlFRG15 | Solyc01g010750 | Stress responsive protein | F: GGATGAAGAGGGATTTCCAA | 120 | 0.96 | 0.995 |
| R: TCAAGAACGTCGGGATTGTA | ||||||
| SlFRG16 | Solyc10g081190 | Leucine-rich repeat receptor-like kinase | F: AACAGGACCAATCCCAAGAG | 147 | 0.99 | 0.995 |
| R: TCAAGTCGAGGATTGTGCTC | ||||||
| SlFRG17 | Solyc04g056350 | Zinc finger family protein | F: ATGCAGCAACGACTAACAGG | 99 | 0.84 | 0.993 |
| R: TGCAAAGAGGACATTCAAGC | ||||||
| SlFRG18 | Solyc01g104700 | Ran protein/TC4 protein ran2b | F: ACTTAGCTGCACAGGCATTG | 125 | 0.91 | 0.989 |
| R: ATGGAAAGGTGCAACAGTGA | ||||||
| SlFRG19 | Solyc10g006480 | Ubiquitin | F: CAACCCTTCACTTGGTCCTT | 91 | 0.86 | 0.988 |
| R: CTTTCCACCTCAAGGGTGAT | ||||||
| SlFRG20 | Solyc10g083570 | Fructose-bisphosphatealdolase | F: TGTTGAGCCTGAGATCCTTG | 136 | 1.01 | 0.992 |
| R: GGGCTTCAACAATGTACCCT | ||||||
| SlFRG21 | Solyc02g089200 | SEP subfamily of the MADS-box gene f | F: GGTGGTGAGCAAAGTCTCAA | 67 | 1.10 | 0.988 |
| R: GAGGTTGGAAGAAACCCTGA | ||||||
| SlFRG22 | Solyc08g081190 | plasma membrane intrinsic protein | F: GACCACTGGATCTTCTGGGT | 99 | 1.39 | 0.999 |
| R: TTAAGACCTGTGGAATGGCA | ||||||
| SlFRG23 | Solyc06g036540 | Unknown | F: GGGCTTATCAAGGAAGGGAT | 138 | 0.86 | 0.992 |
| R: GGCACCTCTTGTCACCTTT | ||||||
| SlFRG24 | Solyc02g080630 | Lactoylglutathionelyase | F: GGATGCAAGTGGTGAAGAAA | 144 | 1.03 | 0.996 |
| R: CGTATGCATTTCCCTTTGTG | ||||||
| SlFRG25 | Solyc05g050200 | Eukaryotic translation initiation factor 1A | F: CGAAGAACAAGGGTAAGGGA | 137 | 0.90 | 0.993 |
| R: ACATCGTCCATTACCAAGCA | ||||||
| SlFRG26 | Solyc01g087320 | tRNA-dihydrouridine synthase A | F: TGTTGCTCCCATGATGAAAT | 97 | 0.94 | 0.994 |
| R: AGCAGCAAGCATTTCTGTGT | ||||||
| SlFRG27 | Solyc06g007510 | Ubiquitin-conjugating enzyme E2 | F: GCTCTCTGTTGACAGACCCA | 108 | 1.10 | 0.998 |
| R: GAGTCCAGCTACGAGCAGTG | ||||||
| SlFRG28 | Solyc06g005060 | elongation factor 1-alpha | F: TTGGTGGTATTGACAAGCGT | 124 | 0.84 | 0.991 |
| R: GTGATACCACGCTCACGTTC | ||||||
| SlFRG29 | Solyc02g084360 | V-type proton ATPase 16 kDaproteo lipid | F: TGCGCCTTTCTTCGGTTTCC | 88 | 0.94 | 0.993 |
| R: CACACCGCTCTTCGCTGTTC | ||||||
| SlFRG30 | Solyc09g082650 | Acireductonedioxygenase | F:GGCAAGAATATGTTGAGACGTTTGTCA | 147 | 0.80 | 0.983 |
| R:CATCAAGAACAGCAAAGCCACCA | ||||||
| SlFRG31 | Solyc12g044600 | NADP-malic enzyme | F: TCAGCGCATATTGCTGCCAA | 125 | 0.90 | 0.996 |
| R: CTGCGGTATGCTGGGCTGTA | ||||||
| SlFRG32 | Solyc01g107870 | Poly(A) RNA binding protein | F: ACCTGGAATGAGGCCTGGTG | 150 | 0.91 | 0.99 |
| R: TGTTGCTGCATCAGCGGAAC | ||||||
| SlFRG33 | Solyc12g055800 | Unknown | F: GCTTCAGACTTGGCCTGTACG | 127 | 0.91 | 1 |
| R: GGCACAAGTTCCACCAAGCA | ||||||
| SlFRG34 | Solyc12g008590 | Acireductonedioxygenase | F: TCGACGATCACCTCCTGTGTG | 81 | 1.12 | 0.962 |
| R: CCCAAACAGCGCCGTCAAG | ||||||
| SlFRG35 | Solyc02g085540 | NADH ubiquinone oxidoreductase | For: CGCCTGGACAGTCCCGTAAA | 127 | 0.87 | 0.998 |
| R: TGGGCCCAAGTTTCAAGGGT | ||||||
| SlFRG36 | Solyc09g009260 | fructose-1,6-bisphosphate aldolase | F: CTGAGTACACCGTCCGTGCT | 102 | 0.90 | 0.996 |
| R: GGTTGACGGTGGCTTCCTCT | ||||||
| SlFRG37 | Solyc11g005330 | Actin | F: GGAGATTGAAACTGCCAGGAGCA | 143 | 1.00 | 0.915 |
| R: CTGCAGCTTCCATACCAATCATGG | ||||||
| SlFRG38 | Solyc01g028810 | chaperonin | F:GTTGTGTGGTGGTTGAGATCAAGG | 74 | 0.99 | 0.915 |
| R:CCGTATCCTGAGTTGTCCATCGG | ||||||
| Solyc01g095050 | Negatively light-regulated protein | No primer | ||||
| Solyc10g074860 | Unknown | No primer |
a
Tomato gene ID in SGN (https://solgenomics.net/). E: Amplification efficiency; R2: Correlation coefficient.