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. 2017 Aug 29;8:1440. doi: 10.3389/fpls.2017.01440

Table 2.

Description of the newly identified RGs, primer sequences, and amplification characteristics.

Gene ID Gene locusa Gene description Primer sequence (5′–3′) Product size (bp) E R2
SlFRG01 Solyc06g005360 Actin depolymerizing factor 3 F: TGACAAAGGAAAGTGTCCCA 133 0.76 0.994
R: ATCTGAATTCCGTCCAGCTC
SlFRG02 Solyc01g088040 Unknown F: GTTGATGAAGGGAGAGCCAT 95 0.78 0.998
R: GAGAGTAGATCACGGCAGCA
SlFRG03 Solyc02g063070 Unknown F: GGCTGAACTGGCTCCTACTC 109 0.77 0.999
R: TTTCGCAAGGTTACAAGCAC
SlFRG04 Solyc12g095990 RNA helicase DEAD40 F: ACTCACGGAGACATGGATCA 107 0.78 0.999
R: TCAATACCACGAGCAAGGAG
SlFRG05 Solyc01g104170 Ankyrin repeat domain-containing protein F: CAAAGGTTGATGCATTGGAC 110 1.05 0.958
R: AGTTACAGCGGCTCCATTCT
SlFRG06 Solyc11g070030 Unknown F: CCACACGACACTTCCATCTC 111 0.90 1
R: TCAGCCGGTTTAGATATCCC
SlFRG07 Solyc01g091150 Golgi SNAP receptor complex member F: CAATCAAGAAGGATCCAGCA 149
R: GCCTTGCCAGTTTCTGTGTA
SlFRG08 Solyc11g042930 E3 ubiquitin ligase F: CTGGCTGCCAACTACTTGAA 71 0.82 0.983
R: TTAATCATGTCTGCCACGGT
SlFRG09 Solyc03g031950 unknown F: CCCAATTTCTTCCTCCGTAA 108 0.98 0.954
R: TGGCCTAAGAAGTCCGAATC
SlFRG10 Solyc10g078450 U6 snRNA-associated Sm-like protein F: TATAGCAATGGAGCAAACCG 112 0.75 0.997
R: TGTCCTCTTTGATGTGCTGA
SlFRG11 Solyc05g023800 Uncharacterized protein F: ACAGGCACTGCATGAAGAAG 109 0.91 0.993
R: GTGAAACTGATCCGCTCTGA
SlFRG12 Solyc04g009770 Ran protein/TC4 protein 1 F: AATCAAGTCCCAACCTGGAG 93 0.90 0.989
R: TTCATGAATGGCCTTTGGTA
SlFRG13 Solyc01g103450 DNAJ chaperone F: AATGACAAGGTTTCCAAGGC 100
R: CTTCTAATCCAGCAATGCGA
SlFRG14 Solyc06g076970 Peptidyl-prolylcis-trans isomerase F: CTCTGCAGTTTGGTCGTGAT 126 0.84 0.983
R: AATACGACCGGCAGGTTTAC
SlFRG15 Solyc01g010750 Stress responsive protein F: GGATGAAGAGGGATTTCCAA 120 0.96 0.995
R: TCAAGAACGTCGGGATTGTA
SlFRG16 Solyc10g081190 Leucine-rich repeat receptor-like kinase F: AACAGGACCAATCCCAAGAG 147 0.99 0.995
R: TCAAGTCGAGGATTGTGCTC
SlFRG17 Solyc04g056350 Zinc finger family protein F: ATGCAGCAACGACTAACAGG 99 0.84 0.993
R: TGCAAAGAGGACATTCAAGC
SlFRG18 Solyc01g104700 Ran protein/TC4 protein ran2b F: ACTTAGCTGCACAGGCATTG 125 0.91 0.989
R: ATGGAAAGGTGCAACAGTGA
SlFRG19 Solyc10g006480 Ubiquitin F: CAACCCTTCACTTGGTCCTT 91 0.86 0.988
R: CTTTCCACCTCAAGGGTGAT
SlFRG20 Solyc10g083570 Fructose-bisphosphatealdolase F: TGTTGAGCCTGAGATCCTTG 136 1.01 0.992
R: GGGCTTCAACAATGTACCCT
SlFRG21 Solyc02g089200 SEP subfamily of the MADS-box gene f F: GGTGGTGAGCAAAGTCTCAA 67 1.10 0.988
R: GAGGTTGGAAGAAACCCTGA
SlFRG22 Solyc08g081190 plasma membrane intrinsic protein F: GACCACTGGATCTTCTGGGT 99 1.39 0.999
R: TTAAGACCTGTGGAATGGCA
SlFRG23 Solyc06g036540 Unknown F: GGGCTTATCAAGGAAGGGAT 138 0.86 0.992
R: GGCACCTCTTGTCACCTTT
SlFRG24 Solyc02g080630 Lactoylglutathionelyase F: GGATGCAAGTGGTGAAGAAA 144 1.03 0.996
R: CGTATGCATTTCCCTTTGTG
SlFRG25 Solyc05g050200 Eukaryotic translation initiation factor 1A F: CGAAGAACAAGGGTAAGGGA 137 0.90 0.993
R: ACATCGTCCATTACCAAGCA
SlFRG26 Solyc01g087320 tRNA-dihydrouridine synthase A F: TGTTGCTCCCATGATGAAAT 97 0.94 0.994
R: AGCAGCAAGCATTTCTGTGT
SlFRG27 Solyc06g007510 Ubiquitin-conjugating enzyme E2 F: GCTCTCTGTTGACAGACCCA 108 1.10 0.998
R: GAGTCCAGCTACGAGCAGTG
SlFRG28 Solyc06g005060 elongation factor 1-alpha F: TTGGTGGTATTGACAAGCGT 124 0.84 0.991
R: GTGATACCACGCTCACGTTC
SlFRG29 Solyc02g084360 V-type proton ATPase 16 kDaproteo lipid F: TGCGCCTTTCTTCGGTTTCC 88 0.94 0.993
R: CACACCGCTCTTCGCTGTTC
SlFRG30 Solyc09g082650 Acireductonedioxygenase F:GGCAAGAATATGTTGAGACGTTTGTCA 147 0.80 0.983
R:CATCAAGAACAGCAAAGCCACCA
SlFRG31 Solyc12g044600 NADP-malic enzyme F: TCAGCGCATATTGCTGCCAA 125 0.90 0.996
R: CTGCGGTATGCTGGGCTGTA
SlFRG32 Solyc01g107870 Poly(A) RNA binding protein F: ACCTGGAATGAGGCCTGGTG 150 0.91 0.99
R: TGTTGCTGCATCAGCGGAAC
SlFRG33 Solyc12g055800 Unknown F: GCTTCAGACTTGGCCTGTACG 127 0.91 1
R: GGCACAAGTTCCACCAAGCA
SlFRG34 Solyc12g008590 Acireductonedioxygenase F: TCGACGATCACCTCCTGTGTG 81 1.12 0.962
R: CCCAAACAGCGCCGTCAAG
SlFRG35 Solyc02g085540 NADH ubiquinone oxidoreductase For: CGCCTGGACAGTCCCGTAAA 127 0.87 0.998
R: TGGGCCCAAGTTTCAAGGGT
SlFRG36 Solyc09g009260 fructose-1,6-bisphosphate aldolase F: CTGAGTACACCGTCCGTGCT 102 0.90 0.996
R: GGTTGACGGTGGCTTCCTCT
SlFRG37 Solyc11g005330 Actin F: GGAGATTGAAACTGCCAGGAGCA 143 1.00 0.915
R: CTGCAGCTTCCATACCAATCATGG
SlFRG38 Solyc01g028810 chaperonin F:GTTGTGTGGTGGTTGAGATCAAGG 74 0.99 0.915
R:CCGTATCCTGAGTTGTCCATCGG
Solyc01g095050 Negatively light-regulated protein No primer
Solyc10g074860 Unknown No primer
a

Tomato gene ID in SGN (https://solgenomics.net/). E: Amplification efficiency; R2: Correlation coefficient.