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. Author manuscript; available in PMC: 2017 Sep 3.
Published in final edited form as: J Gene Med. 2012 May;14(5):299–315. doi: 10.1002/jgm.2626

Figure 1.

Figure 1

Schematic representation of the integration-deficient lentiviral vectors. Schematic depicts linear form of double stranded DNA after completion of reverse transcription. All vectors contain an eGFP expression cassette under the control of a CMV promoter and including a WPRE. The cassettes are flanked by the self-inactivating 5’ deleted LTR. (A) Lenti-LINGO1-sh1-4/Lenti-Scr contain an shRNA expression cassette driven by the H1 promoter placed downstream of the eGFP expression cassette. (B) Lenti-H1 contains the H1 promoter but no shRNA or Pol III termination sequence. dLTR, deleted long terminal repeat; GLS, gag leader sequence incorporating the packaging element (y); RRE, rev response element; cPPT/cTS, central polypurine tract and central termination sequence; CMV, cytomegalovirus immediate early promoter; eGFP, enhancer green fluorescent protein; H1, H1-RNA promoter; shRNA, short hairpin RNA; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element.