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. Author manuscript; available in PMC: 2017 Sep 3.
Published in final edited form as: J Gene Med. 2012 May;14(5):299–315. doi: 10.1002/jgm.2626

Figure 3.

Figure 3

Lenti-LINGO1-sh4 knocks down LINGO-1 mRNA, although non-specific silencing was also observed with the control vectors. (A) Quantification of the relative level of LINGO-1 mRNA in CGNs transduced with lentiviral vectors at an MOI 50. Lenti-LINGO1-sh4 significantly knocked down LINGO-1 expression 2.1 fold and 2.2 fold respectively compared to Lenti-Scr and Lenti-H1 (* P < 0.05). In addition, significantly lower levels of LINGO-1 were detected in CGNs transduced with any of the lentiviral vectors compared to the NVC (*** P < 0.001). Values represent mean and SEM, analysis was performed using one way ANOVA with Tukey post-hoc tests, n = 4/group. (B) Quantification of the relative level of LINGO-1 mRNA in CGNs after transduction at an MOI 10. Lenti-LINGO1-sh4 significantly knocked down LINGO-1 expression 2.2 fold and 2.3 fold respectively compared to Lenti-Scr, Lenti-H1 (* P < 0.05). However, significantly lower levels of LINGO-1 were detected in CGNs transduced with any of the lentiviral vectors compared to the NVC (*** P < 0.001). Values represent mean and SEM, analysis was performed using one way ANOVA with Tukey post-hoc tests, n = 3/group.