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. Author manuscript; available in PMC: 2017 Sep 3.
Published in final edited form as: J Gene Med. 2012 May;14(5):299–315. doi: 10.1002/jgm.2626

Figure 5.

Figure 5

OAS1 and PKR induction following transduction with integration-deficient lentiviral vectors encoding shRNAs. (A) Quantification of the relative level of OAS1 mRNA in CGNs transduced using an MOI 10. There was a significant increase in OAS1 expression with Lenti-LINGO1-sh1, Lenti-LINGO1-sh2 and Lenti-LINGO1-sh4 compared to the NVC. Values represent mean and SEM, analysis was performed using one way ANOVA with Tukey post-hoc tests, * P < 0.05, ** P < 0.01, n = 3/group. (B) Quantification of the relative level of OAS1 mRNA in CGNs transduced using an MOI 50. All the lentiviral vectors significantly increased OAS1 expression compared to the NVC. Values represent mean and SEM, analysis was performed using Kruskal-Wallis with Mann-Whitney post-hoc tests, * P < 0.05, n = 4/group. (C) Quantification of the relative level of PKR mRNA in CGNs transduced using an MOI 10. PKR expression was not significantly affected compared to the NVC. Values represent mean and SEM, analysis was performed using one way ANOVA, P > 0.05, n = 3/group. (D) Quantification of the relative level of PKR mRNA in CGNs transduced using an MOI 50. PKR expression was significantly increased in CGNs transduced with any of the shRNA expressing lentiviral vectors compared to the NVC. Values represent mean and SEM, analysis was performed using Kruskal-Wallis with Mann Whitney post-hoc tests, * P < 0.05, n = 4/group. (E) Quantification of the relative level of Mx1 mRNA in CGNs transduced at an MOI 10. No significant difference in Mx1 expression was observed compared to the NVC. Values represent mean and SEM, analysis was performed using one way ANOVA, P > 0.05, n = 3/group. (F) Quantification of the relative level of Mx1 mRNA in CGNs transduced using an MOI 50. Mx1 expression was not significantly affected compared to the NVC. Values represent mean and SEM, analysis was performed using Kruskal-Wallis, P > 0.05, n = 4/group.