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. Author manuscript; available in PMC: 2017 Sep 3.
Published in final edited form as: Gene Ther. 2015 Dec 10;23(2):231–236. doi: 10.1038/gt.2015.103

Figure 1. Transduced neurons expressing mCherry and eGFP are detected in the contralateral spinal cord following intracortical AAV injection.

Figure 1

(a) Schematic representation of the rat CST: black represents the non-transduced CST and yellow represents the transduced CST. (b) Transduction of the injected cortex, including corticospinal neurons. Scale bar: 1000 µm. Inset shows transduced cortical neurons. Scale bar: 70 µm. Transduced neurons were also observed in regions of the brain that receive direct connections from the cortex including (c) contralateral cortex, Scale bar: 70 µm (d) claustrum nucleus, Scale bar: 250 µm (e) Thalamic regions, Scale bar: 250 µm (f) substantia nigra, Scale bar: 250 µm (g) Numerous mCherry and eGFP positive axons are observed in the contralateral dorsomedial CST. Spinal neurons positive for mCherry and eGFP are observed in the contralateral grey matter. Scale bar: 250 µm. Inset shows higher-magnification images with NeuN staining. Scale bar: 30 µm.