Table 1. Steady-state kinetic parameters of wild-type M.EcoRV and the K16R variant with the adenine (RV_ME) and cytosine substrates (RV_C/A)^a.

M.EcoRV variant	Adenine substrate				Cytosine substrate
	KMAdoMet	KMDNA	kcat	kcat/KMDNA	KMAdoMet	KMDNA	kcat	kcat/KMDNA
	[µM]	[µM]	[h–1]	[µM–1 h–1]	[µM]	[µM]	[h–1]	[µM–1 h–1]
Wildtype	9.9	0.46	2.3	5.0	8.6	0.56	0.09	0.16
K16R	10	1.0	0.10	0.1	8.5	1.1	0.14	0.13

^aK_M and k_cat values are valid ± 20% and ± 30%, respectively.