Table 2. Methylation of cytosine residues located in a GCTATC/G^mATAGC site by M.EcoRV and the K16R, Y196W and K16R/Y196W variants.

M.EcoRV variant	DNA methylation [CPM]
Wild-type	306 ± 20
K16R	78 ± 5
Y196W	333 ± 5
K16R/Y196W	27 ± 2
No enzyme	25 ± 5

In each case, 0.5 µM RV_CG was incubated for 1 h at ambient temperature with 1 µM of the variants.