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. 2017 Aug 30;8:1050. doi: 10.3389/fimmu.2017.01050

Figure 4.

Figure 4

Apoptosis and necrosis in the CNS after cardiopulmonary bypass/deep hypothermic circulatory arrest and ANXA1sp or vehicle treatment. (A) Terminal deoxynucleotidyl nick-end labeling staining and quantification of apoptotic bodies. Apoptosis was significantly reduced in the cerebral cortex of ANXA1sp-treated rats on day 1 post operation. (B,C) Necrosis was detected by acid fuchsin–celestine. Necrosis was reduced in a time-dependent fashion in the hippocampus and cerebral cortex of ANXA1sp rats, with levels returning to baseline by day 7 post operation. Arrowheads in panel A identify pyknotic positive cells. Scale bar: 20 µm. Data are presented as mean ± SD (n = 5–10 slides/tissue section from five rats per group). *P < 0.05, **P < 0.01 compared to vehicle controls, analyzed with unpaired t test.