Figure 5.

Modulation of cerebral NF-κB DNA binding activity and expression of ANXA1 after cardiopulmonary bypass/deep hypothermic circulatory arrest. (A) NF-κB DNA binding activity was significantly reduced in brain homogenates from ANXA1sp-treated rats at 6 h post reperfusion. (B) Co-localization of ANXA1 and NF-κB p65 (arrowheads) was visualized by double immunofluorescence staining and confocal microscopy. (C) ANXA1sp promoted expression of cerebral ANXA1 in rats at 24 h following CPB/DHCA. Scale bar: 20 µm. Data are presented as mean ± SD (n = 3–5 rats/group). *P < 0.05, **P < 0.01 compared to vehicle controls, analyzed with unpaired t test (A) or one-way ANOVA with Tukey’s multiple comparisons test (C).