Figure 6.

Modulation of NF-κB activation after oxygen–glucose deprivation in BV2 cells pretreated with 30 µM ANXA1sp or vehicle. (A) NF-κB activity based on the gel electrophoresis mobility shift assay was significantly reduced in a time-dependent fashion in ANXA1sp-treated cells. (B) After 24 h reoxygenation, co-localization of annexin A1 (ANXA1) and NF-κB p65 subunits was visualized by double immunofluorescence staining and confocal microscopy. Scale bar: 20 µm. Data are presented as mean ± SD (n = 3 independent experiments). ***P < 0.001 compared to vehicle controls, analyzed with unpaired t test.