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Experimental scheme for measuring the hemolymph glucose concentration of honeybees. Honeybees were fed to satiation (A, sated), fed with 4 μl 30% (w/v) sucrose solution (0.88 M) (B, 1 drop), or remained unfed (C, hungry) 15 min before they were systemically injected with octopamine, epinastine, or PBS (injection). Fifteen minutes following the injection hemolymph glucose concentration was measured.
