Figure 4.

Sleep (5 min of inactivity) was quantified for 32 animals per genotype over 12 days of activity induction and compared to stationary controls (LD12:12, lights on 8 am:ZT0) (A–D). Sleep episodes were categorized into night sleep (22 pm–4 am) and siesta sleep (12 am–4 pm) as well as resting phases during morning (4 am–12 am) and evening activity (4 pm–10 pm). Bar graphs show percentage of time spent sleeping during the night, morning, siesta and evening of exercising flies in comparison to stationary controls. (A) Exercising Oregon-R flies spent more time sleeping during the evening compared to non-exercising control (*p < 0.05). (B) Elav-GAL4 flies undergoing activity induction displayed more resting phases than control group in the morning (*p < 0.05) and in the evening (**p < 0.01). (C) UAS-Aβ42/+ flies showed less sleep during activity induction (*p < 0.05) and more activity in the evening (**p < 0.05). (D) In exercising AD flies significantly more sleep was measured during the night (***p < 0.001) followed by less resting phases in the morning (*p < 0.05).