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. 2017 Aug 30;11:159. doi: 10.3389/fnbeh.2017.00159

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Copyright © 2017 Berlandi, Lin, Ambrée, Rieger, Paulus and Jeibmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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For quantification of average locomotor activity, sleep as well as fragmented sleep, DAMs were loaded with 32 animals per genotype in the beginning of the experiment. (A–C) Relative activity of AD flies (elav-GAL4 > UAS-Aβ42) was assessed by normalization to Oregon-R elav-GAL4 and UAS-Aβ42/+ control. Total number of movement counts were averaged for each day and displayed over a period of 20 days in relation to control groups. Activity of controls is considered as 100%. (A) Locomotor activity of AD flies was increased from day 1 to 12 (*p < 0.05) and decreased from day 13 to 20 (***p < 0.001) compared to Oregon-R strain. (B) Relative activity of AD flies was increased from day 1 to 12 (***p < 0.01) compared to elav-GAL4 driver strain. (C) Relative activity of AD flies was increased from day 1 to 12 (***p < 0.01) and reduced from day 13 to 20 (**p < 0.001) compared to UAS-Aβ42/+ control. (D–F) Relative sleep quantity of AD flies was assessed by normalization to Oregon-R, elav-GAL4 and UAS-Aβ42/+ control. Total sleep duration was averaged for each day and is shown over 20 days of data acquisition in relation to control groups. Sleep duration of controls is considered as 100%. Daily sleep in AD flies was decreased from day 1 to 12 compared to elav-GAL4 (**p < 0.01) and increased in comparison to Oregon-R control from day 13 to 20 (**p < 0.01). AD flies showed less sleep from day 1 to 12 (***p < 0.001) and elevated daily sleep from day 13 to 20 (***p < 0.001) compared to UAS-Aβ42/+ flies. (G–I) Relative fragmented sleep (short term sleep) of AD flies was assessed by normalization to Oregon-R, elav-GAL4 and UAS-Aβ42/+ control.Total short term sleep was averaged for each day and is shown over 20 days of experiment in relation to control groups. Fragmented sleep of controls is considered as 100%. Short term sleep quantity of AD flies was increased over a period of 20 days when compared to Oregon-R (***p < 0.001), elav-GAL4 (***p < 0.001) and UAS-Aβ42/+ control flies (***p < 0.001).