Kinetics of digestion with
DNase I. (A) Percentage of matrix-attached DNA
as a function of DNase I concentration. Each point is the mean of
three independent experiments. Error bars show standard deviations.
(B) Agarose gel electrophoresis of DNA isolated
from nuclear matrix prepared with different concentrations of DNase
I. Lane 1, 1 kb DNA ladder; lane 2, control DNA from permeabilized
cells undigested with DNase I; lanes 3–6, DNA from cells
treated with 5, 10, 20 and 40 U/ml DNase I.