Table 1.
Particle size, Zeta (ζ)-potential, polydispersity index, and encapsulation effiency of SOD1 antisense oligonucleotide calcium phosphate-lipid nanoparticles (SOD1-ASO CaP-lipid NPs) prepared at different CaCl2, and Na2HPO4 concentrations.
| CaCl2 (mM) | 100 | 250 | 500 | 500 | 500 |
|---|---|---|---|---|---|
| Na2HPO4 (mM) | 5 | 5 | 5 | 10 | 25 |
| CaP Ratio | 20/1 | 50/1 | 100/1 | 1/50 | 1/20 |
| No. Size (nm) | 61.0 ± 13.7 | 52.3 ± 14.7 | 31.0 ± 2.2 | 39.9 ± 3.3 | 50.4 ± 7.6 |
| Zeta Potential (mV) | −6.3 ± 0.2 | −7.2 ± 0.2 | −4.9 ± 0.1 | −6.6 ± 0.51 | −6.4 ± 0.25 |
| Polydispersity Index (PDI) | 0.2 ± 0.019 | 0.2 ± 0.005 | 0.3 ± 0.006 | 0.2 ± 0.014 | 0.2 ± 0.007 |
| Encapsulation Effiencicy (%) | 69.1 ± 2.7 | 51.1 ± 2.56 | 47.9 ± 5.6 | 34.3 ± 4.15 | 16.1 ± 2.07 |
| Particle Concentration (per mL) | 0.7 E11 | 2.6 E11 | 11.9 E11 | 2.6 E11 | 5.3 E11 |
Data is presented as the average ± SD (n = 5).