Figure 3. The antioxidative effect of TMZ is involved in the TMZ-mediated neuroprotective effect on RGCs.

(A,B) After OGD/R exposure, primary RGCs were subjected to ROS overproduction (green fluorescence). TMZ could significantly decrease ROS levels in RGCs. (C,D) As marked by the peak area of FITC+ population, the proportion of cells with excess ROS was significantly decreased from 80% (±6.1%) to 30% (±1.7%) in the OGD/R + TMZ RGCs. (E,F) OGD/R decreased the MMP, which was marked by a fluorescence shift from red to green in RGCs. TMZ pretreatment elevated the PE/FITC ratio. Scale bar =50 μm. The data represent the means ± S.D. (n=8); **P<0.01.