Figure 5.

Protective effect of CpG-A₁₅₈₅ on lipopolysaccharide (LPS)-induced endotoxin shock. (A) Experimental schedule to examine the effect of CpG-A₁₅₈₅ on LPS-induced endotoxin shock. BALB/c mice were intraperitoneally injected with 300 µg CpG-A₁₅₈₅ 3 h before challenge with 750 µg LPS. (B) Survival and (C) changes in body temperature were monitored for 24 h. (D) Kinetics of platelet-activating factor acetylhydrolase (PAF-AH) activity in serum collected from the tail vein after receiving 300 µg CpG-A₁₅₈₅ and 750 µg LPS. (E) Serum interleukin (IL)-6 and (F) tumor necrosis factor (TNF)-α levels in mice that received LPS with or without 300 µg CpG-A₁₅₈₅ 12 h after LPS challenge. N = 8 mice per group. Data are presented as the mean ± SE. *p < 0.05 and **p < 0.01, significant differences between the LPS and CpG-A₁₅₈₅ + LPS groups.