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. 2017 Aug 30;8:1031. doi: 10.3389/fimmu.2017.01031

Figure 5.

Figure 5

MHC class I-related protein 1 (MR1)-tetramer staining identifies mucosal-associated invariant T cells within the CD161++ Vα7.2+ T cell population. (A) Representative plots showing cells staining for 5-OP-RU MR1-tetramer (top) or Vα7.2 antibody (bottom) within CD4+, CD8+, and double-negative (DN) T cells. Cells are from the same donor, stained in parallel. Frequency of cells within CD4+, CD8+, or DN T cells shown in (B). **P < 0.01 or *P < 0.05, or non-significant = ns by repeated measures one-way ANOVA with Dunnett’s multiple comparisons test, compared to CD8+ T cells (n = 9). (C) Same cells as (A), showing coreceptor expression on CD161++ T cells stained with 5-OP-RU MR1-tetramer (top) or Vα7.2 antibody (bottom). (D) Coreceptor expression on CD161++ MR1-tetramer+ cells (top) and CD161++ Vα7.2+ T cells (bottom). ****P < 0.0001, ***P < 0.001 by repeated measures one-way ANOVA with Dunnett’s multiple comparisons test, compared to CD8+ T cells (n = 9).