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. 2017 Jun 12;36(34):4952. doi: 10.1038/onc.2017.190

Figure 1.

Figure 1

(a) Plasma miR-10b levels. Compared with plasma from normal controls (n=18; closed circles) and chronic pancreatitis patients (CP; n=5; gray circles), miR-10b levels were significantly elevated (P=0.002 and P=0.067, respectively) in the plasma of PDAC patients (n=18; open circles). Horizontal bars denote mean levels. For each sample, 3 μl of a 5 nM cel-miR-39 oligonucleotide stock were added to 100 μl of plasma. RNA was then isolated with TRIzol LS Reagent, and 10 ng of RNA per reaction was converted to cDNA using the TaqMan MicroRNA Reverse Transcription Kit, and TaqMan primers for hsa-miR-10b, hsa-miR-16 and cel-miR-39. qRT-PCR was performed using the ViiA7 as described in Methods. Ct values for hsa-miR-10b were normalized to cel-miR-39 spike-in recovery, then to the internal reference (hsa-miR-16), yielding the dCt. The average dCt for the normal samples was then subtracted from each individual dCt for miR-10b, yielding the ddCt value. Fold increases were calculated relative to miR-10b levels in normal control plasma.