Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 24;36(34):4887–4900. doi: 10.1038/onc.2017.72

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Macmillan Publishers Limited, part of Springer Nature.

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

Generation of embryonic-like stemness of PGCCs. (a) Immunofluorescence photos of OCT4, SSEA1, SOX2 and NANOG in spheroids derived from Hey-derived PGCCs cultured in stem cell medium and reattached on coverslips at different time points. sD, spheroid day. Scale bars, 50 μm. (b) Hematoxylin–eosin (H&E) staining of spheroids from regular Hey cells and PGCCs at sD7 in stem cell medium. Bottom panels are enlarged views of the boxed areas. Black arrow, anaplastic nuclei in a cyst; orange arrow, erythrocyte-like cell in cyst; blue arrow, macrophage-like cell. Scale bars, 50 μm. (c) IHC (colored by DAB, brown) showed the expression of cytokeratin (CK), fibronectin and OCT3/4 in spheroids derived from regular Hey cells and Hey-derived PGCCs. Black arrow indicates rare positive OCT3/4 cells in the middle of spheroids. Scale bars, 50 μm. (d) Immunofluorescence photos of OCT4, NANOG, SSEA1, SOX4 and SOX2 in regular MDA-HGSC-1 cells and spheroids derived from MDA-HGSC-1-derived PGCCs on recovery day 7 (sD1) in stem cell medium. Scale bars, 50 μm. (e) Immunofluorescence photos of Xist in regular Hey and MDA-HGSC-1 cells, PGCCs derived from these cells, and PGCC-derived daughter cells. Boxed regions in the two daughter panels are daughter cells with high magnification, indicated by white arrowheads. Bars, 20 μm. (f) Quantification of OCT4, NANOG and DAZL by qRT-PCR analysis in Hey and SKOV3 cells allowed recovering in regular medium at different times after paclitaxel treatment. (g) Expression of OCT4, NANOG and SOX2 in Hey- and SKOV3-derived PGCCs at different times after paclitaxel treatment, detected by western blotting.