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. 2017 May 8;36(35):5045–5057. doi: 10.1038/onc.2017.118

Figure 4.

Figure 4

Co-implantation of 4T1 cells with CYP4A10high macrophages promotes lung pre-metastatic niche formation and metastasis. 4T1 cells (6 × 105) mixed with wild-type RAW264.7 cells, negative control lentivirus (LV-NC)-treated RAW264.7 cells or CYP4A10high RAW264.7 cells (2 × 105) were injected into the right mammary fat pads of BALB/c mice in a ratio of 3:1. (a) Hematoxylin/eosin-stained lung sections and quantification of lung metastase on day 28 after tumor implantation (n=8). Scale bars, 5 mm. (b) The tumor weight was measured on day 14 and day 28 after tumor implantation (n=8). (c) 20-HETE production in TAMs on day 14 or day 28 were determined by LC-ESI-MS/MS (n=8). (d) VEGFR1+ myeloid cells in the pre-metastatic lungs were analyzed by flow cytometry on day 14 (n=5). (e) MMP-9, S100A8 and fibronectin in the pre-metastatic lungs were determined by western blot (n=8). The value is presented as the mean±s.e.m. *P<0.05, **P<0.01 vs LV-NC.