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. 2017 May 8;36(35):5045–5057. doi: 10.1038/onc.2017.118

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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CYP4A inhibition regulates TAM polarization away from the M2 phenotype in 4T1 breast cancer and B16F10 melanoma. (a) Immunostaining analysis of F4/80⁺CD206⁺ macrophages in the tumor tissues from the groups treated with or without HET0016 and DDMS was shown. Scale bars, 20 μm. (b) Corresponding quantitative analysis of tumor-infiltrating F4/80⁺ macrophages and F4/80⁺CD206⁺ M2 macrophages (n=8). (c) Relative mRNA levels of M1 marker (iNOS) and M2 markers (CD206 and arginase-1) in TAMs were measured by qPCR (n=8). (d) Relative protein levels of M1/M2 markers in TAMs were determined by western blot (n=8). (e) Production of Th1 cytokines (TNF-α and IL-12) and Th2 cytokines (TGF-β and IL-10) in TAMs was determined by ELISA (n=8). The value is presented as the mean±s.e.m. *P<0.05, **P<0.01 vs model.