Figure 12.

ARF interacts with FAK in H1299 cells. (a) H1299 cells were transiently transfected with empty vector (v) or either p53 or DAPK-expressing plasmids. After 24 h, cells were re-plated and cytoplasmic extracts immunoprecipitated with anti-ARF antibody and analyzed by western blot with anti-pFAK Y397, ARF and p53 antibodies. Panels of input are also shown on the right of immunoprecipitation experiment. (b) H1299 cells were transfected with an empty vector (v) or increasing amount of wt DAPK or DAPK K42A expression plasmids. Twenty-four hours after transfection, cells were lysed and cellular extracts were immunoblotted and analyzed with anti-FAK, anti-DAPK and anti-tubulin as loading control. WB shown are representative of at least three independent experiments. Normalized FAK band intensities are shown below each corresponding band, and are expressed as fold enrichment with respect to empty vector-transfected sample arbitrarily set to 1.