Skip to main content
. 2017 Apr 24;36(34):4913–4928. doi: 10.1038/onc.2017.104

Figure 5.

Figure 5

Both human and mouse ARF transfections rescue cell spreading defect of p14ARF-depleted cells. HeLa cells transiently transfected with empty, flag-ARF or p19ARF vector for 24 h were treated with the indicated siRNAs for 48 h, detached and re-plated as described above. (a) Western blot analysis with anti-Flag, ARF (human and mouse) and actin from collected cells. (b) Images of re-plated cells acquired by phase-contrast microscope (scale bar, 30μm). (c) Cumulative data are expressed as a mean value±s.e.m. of at least three independent experiments. Single experiment data have been tested as described in Materials and methods section to analyze normal distribution. Asterisks (*) indicate statistically significant differences by paired two-tailed Wilcoxon test. *P<0.001 and **P<0.01 by paired Student's t-test. Number of cells analyzed for each experiment: SCR siRNA+ empty vector (350), ARF siRNA+empty vector (330), ARF siRNA+Flag-ARF (300), SCR siRNA+Flag-ARF (350), SCR siRNA+p19ARF (336) and ARF siRNA+p19ARF (300).