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. 2017 Aug 24;5(16):e13291. doi: 10.14814/phy2.13291

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© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Evaluation of the levels of MuRF‐1 and MAFbx mRNA (A) and MuRF‐1 protein (B) in soleus muscles of C, HS, and HS+IMD rats. A: Evaluation of MuRF‐1 and MAFbx mRNA expression by quantitative PCR. Values are normalized to the levels of β‐actin mRNA in each sample. B: Evaluation of MuRF‐1 protein expression by Western blotting. Values are normalized to the levels of total protein and GAPDH in each sample. N = 7. * indicates a significant difference from control, P < 0.05; # indicates a significant difference from HS, P < 0.05. Western blot image was cropped to display a single band with predicted molecular weight that was identified in our experiments. Abcam web page displays a Western blot where the same anti‐MuRF‐1 antibody recognizes a single 40 kDa band.