Figure 3.

(A) Paired extensor digitalis longus muscles were subjected to basal and insulin‐stimulated 2‐DG uptake measurements as described in Methods. Uptake values are means  ± SEM from 4 LF + SH, 4 HF + SH, and 5 HF + EX muscles. (B, C, D) Mix hindlimb skeletal muscle were subjected to fractionation for membrane cholesterol determination (B) or were labeled with antibodies against F‐actin and imaged by confocal microscopy (C) and images were digitally quantitated using MetaMorph software (D) as described in Methods. Cholesterol values are means ± SEM from 12 LF + SH, 12 HF + SH, and 12 HF + EX muscles and F‐actin values are means ±SEM from 3 to 5 images of 5 LF + SH, 5 HF + SH, and 5 HF + EX muscles. One‐way ANOVA post hoc analysis statistics are indicated with *P < 0.05 (vs. LF + SH +Insulin); #P < 0.05 (vs. HF + SH + Insulin).