Figure 6.

Creatine supplementation enhances HIF‐1 activity in RNCMs subjected to hypoxia. HIF‐1 function was quantified using a luciferase reporter in RNCM cultures supplemented with 0.5 mmol/L AICAR, 1 mmol/L Cr, or both for 24 h prior to 12 h incubation in hypoxic conditions, as described in Materials and Methods. Each bar represents the mean ± SEM of four independent experiments, normalized to control. An “*” denotes a statistically significant difference compared with control normoxia and a “+” denotes a statistically significant difference compared to control hypoxia (two‐way ANOVA, P < 0.05, Tukey's test).