Table 2.
C | FF | n | P value | |
---|---|---|---|---|
Mitochondrial complex | ||||
Complex I (μmol min−1 mg−1) | 0.58 ± 0.03 | 0.71 ± 0.04 | 8 | 0.021 |
Complex II (μmol min−1 mg−1) | 0.17 ± 0.01 | 0.13 ± 0.01 | 8 | 0.052 |
Complex III (μmol min−1 mg−1) | 4.00 ± 0.31 | 4.38 ± 0.32 | 8 | 0.410 |
Complex IV (μmol min−1 mg−1) | 6.31 ± 0.50 | 5.27 ± 0.26 | 8 | 0.085 |
CPT activity | ||||
CPT total (μmol min−1 g−1) | 13.14 ± 0.55 | 14.45 ± 1.31 | 7 | 0.372 |
CPT 2 (μmol min−1 g−1) | 7.93 ± 0.72 | 11.75 ± 1.11 | 7 | 0.013 |
CPT 1 (μmol min−1 g−1) | 5.21 ± 0.18 | 2.71 ± 0.60 | 7 | 0.002 |
β‐Oxidation | ||||
LCAD (nmol min−1 mg−1) | 9.93 ± 0.42 | 8.27 ± 0.30 | 6 | 0.009 |
MCAD (nmol min−1 mg−1) | 3.15 ± 0.23 | 4.35 ± 0.18 | 6 | 0.002 |
β‐HCAD (nmol min−1 mg−1) | 3.18 ± 0.34 | 4.31 ± 0.32 | 8 | 0.029 |
Other | ||||
Aconitase (μmol min−1 mg−1) | 96.4 ± 9.4 | 46.1 ± 4.8 | 8 | 0.0005 |
ALDH2 (nmol min−1 mg−1) | 626 ± 83 | 263 ± 42 | 6 | 0.003 |
Citrate Synthase (μmol min−1 mg−1) | 0.96 ± 0.03 | 0.81 ± 0.02 | 6 | 0.003 |
PDH activity (mOD min−1 CS−1) | 100.9 ± 4.6 | 83.4 ± 4.4 | 7 | 0.017 |
SOD, mitochondrial (U mg−1) | 3.87 ± 0.54 | 3.07 ± 0.24 | 6 | 0.244 |
SOD, cytosolic (U mg−1) | 203 ± 13 | 228 ± 21 | 6 | 0.339 |
Data are presented as mean ± SE. Bold indicates the results significant at the 0.05 level.
C, age‐matched control rats (fed standard chow and water); FF, rats fed standard chow and 10% fructose added to the drinking water; β‐HCAD, β‐hydroxyacyl‐CoA dehydrogenase; MCAD, acyl‐CoA dehydrogenase, medium chain; LCAD, acyl‐CoA dehydrogenase, long chain; ALDH2, aldehyde dehydrogenase‐2; CPT, carnitine palmitoyltransferase; CS, citrate synthase; PDH, pyruvate dehydrogenase; SOD, superoxide dismutase.