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. 2017 Aug 22;5(16):e13388. doi: 10.14814/phy2.13388

Table 2.

Enzymatic activities

C FF n P value
Mitochondrial complex
Complex I (μmol min−1 mg−1) 0.58 ± 0.03 0.71 ± 0.04 8 0.021
Complex II (μmol min−1 mg−1) 0.17 ± 0.01 0.13 ± 0.01 8 0.052
Complex III (μmol min−1 mg−1) 4.00 ± 0.31 4.38 ± 0.32 8 0.410
Complex IV (μmol min−1 mg−1) 6.31 ± 0.50 5.27 ± 0.26 8 0.085
CPT activity
CPT total (μmol min−1 g−1) 13.14 ± 0.55 14.45 ± 1.31 7 0.372
CPT 2 (μmol min−1 g−1) 7.93 ± 0.72 11.75 ± 1.11 7 0.013
CPT 1 (μmol min−1 g−1) 5.21 ± 0.18 2.71 ± 0.60 7 0.002
β‐Oxidation
LCAD (nmol min−1 mg−1) 9.93 ± 0.42 8.27 ± 0.30 6 0.009
MCAD (nmol min−1 mg−1) 3.15 ± 0.23 4.35 ± 0.18 6 0.002
β‐HCAD (nmol min−1 mg−1) 3.18 ± 0.34 4.31 ± 0.32 8 0.029
Other
Aconitase (μmol min−1 mg−1) 96.4 ± 9.4 46.1 ± 4.8 8 0.0005
ALDH2 (nmol min−1 mg−1) 626 ± 83 263 ± 42 6 0.003
Citrate Synthase (μmol min−1 mg−1) 0.96 ± 0.03 0.81 ± 0.02 6 0.003
PDH activity (mOD min−1 CS−1) 100.9 ± 4.6 83.4 ± 4.4 7 0.017
SOD, mitochondrial (U mg−1) 3.87 ± 0.54 3.07 ± 0.24 6 0.244
SOD, cytosolic (U mg−1) 203 ± 13 228 ± 21 6 0.339

Data are presented as mean ± SE. Bold indicates the results significant at the 0.05 level.

C, age‐matched control rats (fed standard chow and water); FF, rats fed standard chow and 10% fructose added to the drinking water; β‐HCAD, β‐hydroxyacyl‐CoA dehydrogenase; MCAD, acyl‐CoA dehydrogenase, medium chain; LCAD, acyl‐CoA dehydrogenase, long chain; ALDH2, aldehyde dehydrogenase‐2; CPT, carnitine palmitoyltransferase; CS, citrate synthase; PDH, pyruvate dehydrogenase; SOD, superoxide dismutase.