Table 4.
Mitochondrial respirometry data
| State 3 (ADP present) | State 4 (Oligomycin present) | (State 3/State 4) Ratio | |||||||
|---|---|---|---|---|---|---|---|---|---|
| C | FF | P value | C | FF | P value | C | FF | P value | |
| Pyruvate/Malate | 19.6 ± 1.9 | 20.3 ± 1.4 | 0.781 | 1.55 (1.37; 2.39) | 1.25 (1.18; 1.47) | 0.161 | 11.7 ± 1.1 | 14.7 ± 1.3 | 0.0997 |
| Glutamate/Malate | 14.9 ± 2.2 | 13.5 ± 2.5 | 0.674 | 1.13 (0.77; 1.93) | 1.02 (0.93; 1.51) | 0.798 | 12.0 ± 1.0 | 11.6 ± 1.9 | 0.866 |
| Succinate | 16.5 ± 2.2 | 17.8 ± 1.5 | 0.630 | 3.03 (2.20; 5.08) | 3.29 (2.76; 4.23) | 0.721 | 5.3 (3.9; 5.6) | 5.2 (5.1; 5.5) | 1.000 |
| Ascorbate/TMPD | 35.7 ± 4.1 | 47.6 ± 4.7 | 0.079 | — | — | — | — | — | — |
| Pal‐CoA/Car/Malate | 5.4 ± 0.8 | 9.1 ± 0.4 | 0.0008 | 0.92 ± 0.10 | 0.94 ± 0.04 | 0.857 | 5.9 ± 0.8 | 9.8 ± 0.4 | 0.0007 |
| PalCar/Malate | 6.8 ± 1.2 | 7.0 ± 0.9 | 0.875 | 1.10 ± 0.15 | 1.13 ± 0.12 | 0.888 | 6.5 ± 1.0 | 6.5 ± 0.8 | 0.995 |
| OctCar/Malate | 7.9 ± 0.6 | 10.3 ± 0.8 | 0.0296 | 0.97 (0.85; 1.21) | 1.17 (0.95; 1.32) | 0.195 | 7.8 ± 0.7 | 9.1 ± 0.7 | 0.174 |
| AcetylCar/Malate | 9.5 ± 1.0 | 11.6 ± 1.3 | 0.203 | 0.88 ± 0.11 | 0.89 ± 0.11 | 0.981 | 11.2 ± 1.0 | 13.6 ± 0.9 | 0.0961 |
Data are presented as mean ± SE or median (25th; 75th percentile), expressed in pmol O2/(s ug) mitochondria (n = 7–8/group). Bold indicates the results significant at the 0.05 level.
C, age‐matched control rats (fed standard chow and water); FF, rats fed standard chow and 10% fructose added to the drinking water; Pal‐CoA, palmitoyl‐CoA; PalCar, palmitoylcarnitine; OctCar, octanoylcarnitine; AcetylCar, acetylcarnitine; Car, carnitine; TMPD, tetramethyl‐p‐phenylenediamine.