Table 1.
Antibodies and dilutions used in immunoblotting
| Antibodies | Primary dilution | Secondary dilution | Company/Product no |
|---|---|---|---|
| Heat shock protein (HSP70) | 1:1000 | 1:2000 | Enzo Life Sciences: C95F3A‐5 |
| Heat shock protein (HSP60) | 1:750 | 1:2000 | Cell signaling Technology: 12165 |
| Heat shock protein (HSP90) | 1:1000 | 1:3000 | Cell signaling Technology: 4874 |
| Stress‐activated protein kinases (SAPK)/c‐Jun N‐terminal kinases (JNK) | 1:1000 | 1:3000 | Cell signaling Technology: 9251 |
| phospho‐SAPK/JNKThr183/Tyr185 | 1:1000 | 1:2000 | Cell signaling Technology: 9251 |
| Nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB p65) | 1:5000 | 1:3000 | Cell signaling Technology: 8242 |
| phospho‐NF‐κB p65Ser536 | 1:1000 | 1:1000 | Cell signaling Technology: 3033 |
| Nuclear factor kappa‐alpha kinase subunit alpha (IKKα) | 1:1000 | 1:2000 | Cell signaling Technology: 2682 |
| Nnuclear factor of kappa light polypeptide gene enhancer in B‐cells inhibitor, alpha (IKBα) | 1:2000 | 1:2000 | Cell signaling Technology: 9242 |
| Tumor necrosis factor alpha (TNFα) | 1:2000 | 1:2000 | Cell signaling Technology: 3707 |
| Interleukin‐6 (IL‐6) | 1:500 | 1:2000 | Cell signaling Technology: 12153 |
| Interleukin‐1β | 1:500 | 1:2000 | Cell signaling Technology: 12242 |
| Janus Kinase (JAK1) | 1:1000 | 1:2000 | Cell signaling Technology: 3341 |
| Janus Kinase (JAK2) | 1:1000 | 1:2000 | Santa Cruz Biotechnology: SC294 |
| Signal transducer and Activator of Transcription (STAT3) | 1:1000 | 1:3000 | Cell signaling Technology: 4904 |
| phospho‐STAT3Tyr705 | 1:750 | 1:1000 | Cell signaling Technology: 9131 |