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. 2017 Aug 22;5(16):e13397. doi: 10.14814/phy2.13397

Table 2.

Sequences for RT‐qPCR primers

Target Forward primer Reverse primer Ta, °C
18S ctctagataacctcgggccg gtcgggagtgggtaatttgc 60.0
IL‐2 ggtgcacctacttcaagctc ctccctccagagctttgagt 64.4
IL‐6 agatgccaaaggtgatgcca ctcagggtctggatcagtgc 65.7
IL‐1β ccaaagagggacatggagaa ttatatcttggcggcctttg 62.2
IL‐8 gaaatcacaggatgcccagt tgcaagttgaggcaagaaga 61.8
IL‐10 tgtgccctatggtgttcaac ctttgtcacactccggaagc 59.7
IL‐15 tcctggagttacgcgtcatt ttttcctccagctcctcaca 62.2
TNFα gcccttccaccaacgttttc tcccaggtagatgggttcgt 66.9