Table 8.
Gene | Illumina sequencing | Real-time PCR | ||
---|---|---|---|---|
|
|
|||
Fold change1) | q-value2) | Fold change3) | p-value4) | |
ACSL3 | 1.22 | 0.88 | 1.56 | 0.37 |
PPP1R3A | 26.7 | 0.006 | 184.17 | 0.03 |
MTMR7 | 1.17 | 0.99 | 1.42 | 0.30 |
FSHβ | 36.41 | 0.008 | 162.75 | 0.02 |
SMURF2 | 16.83 | 0.03 | 198.49 | 0.02 |
qRT-PCR, real-time quantitative polymerase chain reaction; ACSL3, acyl-CoA synthetase long-chain family member 3; PPP1R3A, protein phosphatase 1 regulatory subunit 3A; MTMR7, myotubularin related protein 7; FSHβ, follicle stimulating hormone beta; SMURF2, SMAD specific E3 ubiquitin protein ligase 2; FPKM, fragments per kilobase of exon per million mapped reads.
Ratio of FPKM values between peak stage and late stage.
False discovery rate, q-value <0.05 was considered as significant.
Relative expression of genes was normalized to the control gene β-actin using 2−ΔΔCt method.
Student’s t-test p-values between peak stage and late stage, p-value <0.05 was considered as significant.