Table 8.

Comparison of the Illumina sequencing expression data and qRT-PCR result for five selected genes

Gene	Illumina sequencing		Real-time PCR
	Fold change1)	q-value2)	Fold change3)	p-value4)
ACSL3	1.22	0.88	1.56	0.37
PPP1R3A	26.7	0.006	184.17	0.03
MTMR7	1.17	0.99	1.42	0.30
FSHβ	36.41	0.008	162.75	0.02
SMURF2	16.83	0.03	198.49	0.02

qRT-PCR, real-time quantitative polymerase chain reaction; ACSL3, acyl-CoA synthetase long-chain family member 3; PPP1R3A, protein phosphatase 1 regulatory subunit 3A; MTMR7, myotubularin related protein 7; FSHβ, follicle stimulating hormone beta; SMURF2, SMAD specific E3 ubiquitin protein ligase 2; FPKM, fragments per kilobase of exon per million mapped reads.

¹⁾Ratio of FPKM values between peak stage and late stage.

²⁾False discovery rate, q-value <0.05 was considered as significant.

³⁾Relative expression of genes was normalized to the control gene β-actin using 2^−ΔΔCt method.

⁴⁾Student’s t-test p-values between peak stage and late stage, p-value <0.05 was considered as significant.