Table 3.

Effect of AFE on bacterial community in rumen fluid at the phylum level

Items	AFE				SEM	p-value
	A	B	C	D		Linear	Quadratic
Bacteroidetes	52.69	46.96	48.10	51.51	6.23	0.93	0.49
Firmicutes	39.10	45.59	41.02	40.42	5.16	0.98	0.52
Proteobacteria	2.70	1.85	3.24	2.16	0.56	0.93	0.84
Tenericutes	1.45	1.71	2.28	1.53	0.26	0.52	0.10
Actinobacteria	0.64	0.94	0.76	0.91	0.29	0.64	0.81
Candidate_division_TM7	0.72	0.84	0.75	0.56	0.16	0.45	0.36
Chloroflexi	0.45	0.35	0.97	0.47	0.26	0.58	0.47
Spirochaetes	0.48	0.32	0.68	0.34	0.17	0.92	0.63
Planctomycetes	0.43	0.35	0.41	0.31	0.10	0.53	0.95
Cyanobacteria	0.29	0.26	0.29	0.24	0.03	0.39	0.87
Euryarchaeota	0.027	0.037	0.042	0.067	0.011	0.04	0.51
Synergistetes	0.21	0.10	0.33	0.13	0.06	0.97	0.49
Fusobacteria	0.29	0.20	0.14	0.13	0.04	0.04	0.41
Lentisphaerae	0.15	0.12	0.12	0.15	0.03	0.84	0.29
Fibrobacteres	0.06	0.08	0.09	0.05	0.02	0.86	0.20
Candidate_division_SR1	0.04	0.04	0.07	0.04	0.01	0.41	0.38

AFE, alfalfa flavonoids extract; SEM, standard error of the mean.