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. 2017 Jul 31;40(8):558–566. doi: 10.14348/molcells.2017.0058

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© The Korean Society for Molecular and Cellular Biology. All rights reserved.

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Fig. 4 — Deformity and functional activity of mitochondria in mature (metaphase II stage) oocytes retrieved after superovulation treatment or natural ovulation.

(A) (natural ovulation) and (B) (superovulation) show morphological difference of mitochondria. Confocal images of mitochondria in oocytes retrieved from natural ovulation (C) or superovulation after JC-1 staining (D). (E) Statistical comparison of mitochondrial activity (mean ± SE) with using of confocal images. Significant (p = 0.0179) difference between two treatments. (F) Quantitative comparison of ATP generation (mean ± SE) in oocytes retrieved from after superovulation or retrieved from natural ovulation. Significantly (p = 0.0002) different. Arrowhead, Well-developed mitochondrial cristae; asterisk, vacuolated mitochondria; F, Filament; L, Lipid droplet; M, Mitochondria. Magnification = ×12,000. Scale bars = 2 μm (A, B)