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Experimental design
| Treatment group | Number of birds | Protein (100 μg/bird) | Adjuvant | Challenge infection (EM+CP1)) |
|---|---|---|---|---|
| 1 | 20 | - | - | - |
| 2 | 20 | - | - | + |
| 3 | 20 | Profilin | - | + |
| 4 | 20 | Profilin+NetB | - | + |
| 5 | 20 | Profilin+NetB | IMS 1142106 | + |
| 6 | 20 | Profilin+NetB | IMS 1142101 | + |
Chickens were orally infected with 1.0×104 oocysts/bird of E. maxima (EM) at day 14 post-hatch and with 1.0×109 CFU/bird of C. perfringens (CP) at day 18.
