Table 2.
Oligonucleotide primers used for quantitative RT-PCR
| RNA target | Primer sequences | PCR product size (base pair) | GenBank accession no. |
|---|---|---|---|
| TNFSF15 | |||
| Forward | 5′-CCTGAGTATTCCAGCAACGCA-3′ | 292 | NM_01024578 |
| Reverse | 5′-ATCCACCAGCTTGATGTCACTAAC-3′ | ||
| IL-8 | |||
| Forward | 5′-GGCTTGCTAGGGGAAATGA-3′ | 200 | NM_205498.1 |
| Reverse | 5′-AGCTGACTCTGACTAGGAAACTGT-3′ | ||
| GAPDH | |||
| Forward | 5′-GGTGGTGCTAAGCGTGTTAT-3′ | 264 | NM_204305.1 |
| Reverse | 5′-ACCTCTGTCATCTCTCCACA-3′ | ||
qRT-PCR, quantitative real time polymerase chain reaction; TNFSF15, tumor necrosis factor superfamily 15; IL-8, interleukin-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.